Search results for "Photoactivated localization microscopy"

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Super-resolved linear fluorescence localization microscopy using photostable fluorophores: A virtual microscopy study

2017

Abstract Current approaches to overcome the conventional limit of the resolution potential of light microscopy (of about 200 nm for visible light), often suffer from non-linear effects, which render the quantification of the image intensities in the reconstructions difficult, and also affect the quantification of the biological structure under investigation. As an attempt to face these difficulties, we discuss a particular method of localization microscopy which is based on photostable fluorescent dyes. The proposed method can potentially be implemented as a fast alternative for quantitative localization microscopy, circumventing the need for the acquisition of thousands of image frames and…

0301 basic medicineMaterials sciencebusiness.industryMultispectral imageResolution (electron density)02 engineering and technology021001 nanoscience & nanotechnologyFluorescenceAtomic and Molecular Physics and OpticsElectronic Optical and Magnetic Materials03 medical and health sciences030104 developmental biologyOpticsMicroscopyCalibrationPhotoactivated localization microscopyElectrical and Electronic EngineeringPhysical and Theoretical Chemistry0210 nano-technologybusinessVirtual microscopyVisible spectrumOptics Communications
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Confocal microscopy of single molecules of the green fluorescent protein

1998

Single molecule detection has been extended into life sciences by use of strongly fluorescent labels. The green fluorescent protein (GFP) as a self-fluorescent biomolecule has attracted considerable attention. Here, single molecules of the GFP-mutant Glu222Gln are immobilized in a polyvinylalcohol matrix and detected by confocal fluorescence microscopy. Although this mutant stabilizes one of both conformers of the wild-type GFP, the investigation of its fluorescence dynamics reveals strong signal fluctuations. This fluorescence behaviour is—at least partly—caused by reversible photochemical changes of the protein framework, that can relax into the fluorescent state on different timescales. …

ChemistryConfocalBiophysicsFluorescence in the life sciencesFluorescencelaw.inventionGreen fluorescent proteinCell biologyBimolecular fluorescence complementationConfocal microscopylawFluorescence microscopeBiophysicsRadiology Nuclear Medicine and imagingPhotoactivated localization microscopyBioimaging
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